Comparison of methods for determining the effectiveness of antibacterial functionalized textiles
نویسندگان
چکیده
Antimicrobial functionalization of textiles is important for various applications, such as protection of textile materials from decomposition, generation of more effective wound dressings, and the prevention of infections or malodors resulting from bacterial growth. In order to test the efficacy of new products, their antibacterial activity needs to be evaluated. At present, several different procedures are being used for this purpose, hindering comparisons among different studies. The present paper compares five of these assays using a sample panel of different textiles functionalized with copper (Cu) and silver (Ag) as antibacterial agents, and discusses the suitability of these methods for different analytical requirements. Bacterial viability was determined by measuring the optical density at 600 nm, a colorimetric assay based on MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) conversion, an agar diffusion assay, and colony formation, either after culturing in media containing textile samples, or after recovery from textiles soaked with bacterial suspension. All experiments were performed with a Gram-negative (Escherichia coli) and a Gram-positive (Staphylococcus warneri) model organism. In general, the results yielded by the different methods were of good comparability. To identify the most suitable test system for the particular type of antibacterial coating, several factors need to be taken into account, such as choosing appropriate endpoints for analyzing passive or active antibacterial effects, selection of relevant microorganisms, correcting for potential interference by leaching of colored textile coatings, required hands on time, and the necessary sensitivity.
منابع مشابه
Effect of Chitosan, Aluminum Oxide and Silver Nanoparticles on Antibacterial, Deodorizing and Moisture Absorption Properties of Nonwoven Polyester Fabrics for Use in Medical Textiles
ABSTRACT Background and Objective: Hospital clothing and textiles have a great role in providing medical services to patients. Hospital services would not be possible without using these textiles. The aim of this study was to prepare non-woven polyester for use in medical textiles. Methods:...
متن کاملSafety and efficacy of antimicrobial nanostructured textiles: comparison with 11070 national standard
Background: This study aimed to evaluate the efficacy and safety of five randomly selected nanostructured products by the National Standard of No. 11070. Materials and methods: Antibacterial activities of five different nanostructured textiles were assessed on specific microorganisms to human skin and environment. The physicochemical properties were determined by FTIR spectrum and the dermal to...
متن کاملSkin-protective effects of a zinc oxide-functionalized textile and its relevance for atopic dermatitis
Atopic dermatitis (AD) is a chronic inflammatory disease characterized by the impairment of the skin-barrier function, increased oxidative cellular stress, and bacterial colonization. Hence, medical therapies of AD aim to control infection, reduce inflammation, and restore skin-barrier function by use of topical and systemic antibacterial drugs, topical corticosteroids, topical calcineurin inhi...
متن کاملQuaternary Ammonium Salts of Poly(N,N-dimethylaminoethyl methacrylate) as an Efficient Antibacterial Agent for Polylactide Textiles
متن کامل
Antibacterial Activity of Textiles for Wound Treatment
Major advances in the development and use of antimicrobial textiles to control bacterial proliferation on wound beds continue. However, wound dressings are, in general, not included in standardized regimens for measuring and monitoring their antimicrobial effectiveness. This work adapts these methods to assess the antibacterial activity of textiles designed for wound healing purposes. Environme...
متن کامل